Stop adding soil once the total sediment depth in the container is between two and three inches deep (5 to 7 cm).After four or five weeks of doing this, you can double the quantity of soil you add per week, as long as the water quality remains healthy. Repeat this once per week, and occasionally test the water quality for ammonia, nitrite, and nitrate. Be careful not to harm the worms you can use a spoon and move it slowly to let the worms get out of the way. After no more than one week has passed, gently stir the clumps of soil into the top half-inch (1.5 cm) of sand and then drop in another clump or two (to keep the worms fed).A reasonable adult Tubifex worm density to aim for once the substrate is healthy, and high quality is about one per square centimeter, which would come to about three or four hundred in a roughly square-shaped 2-gallon plastic container. Also, add a handful of scuds and typical pond snails. Add a few Tubifex worms 50 would be plenty to start with.Neither stir that dirt nor evenly distribute it at this point it needs to stay concentrated to provide a high-quality food source for the invertebrates. Take one or two grape-sized clumps of that dirt (which should be relatively dry) and drop them into the refugium.
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